Training

Hands-On Training in 3D Cell Culture

Get ready to work in 3D – gain practical experience with Real Research’s LifeGel-based 3D models

📌 The training consists of two parts:

🔹Theoretical – up to 30 min (key knowledge about 3D cultures)

🔹Practical – 3-4 hours (hands-on training of essential procedures)

We prioritize quality and a personalized approach, which is why we keep our training groups small (3–4 participants). This ensures that each attendee receives individual support and gains confidence in working with LifeGel.

📋 Training Agenda

Theoretical part:

🔹Introduction to 3D Cell Culture and LifeGel Technology

Key principles of 3D culture, challenges in traditional systems, and how LifeGel offers a reproducible alternative

🔹Overview of Laboratory Procedures

Step-by-step walk-through of the practical session, including purpose and expected outcomes

🔹Q&A Session

Practical part:

🔹Core Techniques Using LifeGel:

Seeding Cells in 3D (LifeGel-based setup)

Learn how to prepare and seed cells onto the LifeGel hydrogel for consistent 3D structure formation

Medium Exchange in 3D Culture

Techniques for efficient and non-disruptive media changes in LifeGel-based system

🔹Hydrogel Digestion: LifeGel Digestion and 3D Structure Retrieval

Methods for retrieving whole 3D structures or isolating single cells for downstream applications (e.g., flow cytometry, RNA extraction, protein isolation)

🔹Assay Example: Cell Viability Assessment

Quantitative approach to evaluating cell viability in 3D models.

🔹Optimization & Wrap-Up:

Troubleshooting and Best Practices

Discussion of common pitfalls and how to optimize results for different cell types or experimental aims.

Final Q&A and Wrap-Up Discussion

⏰ Duration

Approximately 4–5 hours

📍 Location

Real Research headquarters, Kraków, Poland

🔁 Training Schedule

We organize cyclical trainings throughout the year

Individual training sessions can be scheduled upon request for groups of 3 or more participants

To check upcoming training dates or arrange a personalized session, please Contact Us

📜 Certificate

All participants receive a Certificate of Completion issued by Real Research

💰 Price: 1257 pln net/ 307 EUR/362 USD

🛠️ Additional Information

We offer the flexibility to customize the training program based on your specific needs. Upon request, we can incorporate additional techniques or procedures relevant to your research or application area.

Tailored sessions are priced individually, depending on the selected scope, complexity, and included methods. If you have particular expectations or goals, we’ll be happy to prepare a personalized training proposal for your team.

Testimonials

“I had the pleasure of participating in a training organized by Real Research in Kraków, a company specializing in 3D cell culture models. I rate the entire experience very highly, both in terms of content and organization. Special recognition goes to Ms. Ewelina, who oversaw the organizational aspects from the very beginning. Communication with her was quick, efficient, and very helpful. With great kindness and professionalism, she arranged a convenient training date and assisted in resolving all formal matters. The practical part was led by Mr. Marcin and Ms. Ola. This duo impressed not only with their knowledge and experience but also with their genuine commitment to sharing it. Every stage of the work was carefully explained and carried out under the attentive supervision of the instructors, which allowed us to gain practical skills in a comfortable environment. I wholeheartedly recommend this training to anyone who wishes to deepen their knowledge and practice in 3D cell culture. Real Research guarantees quality, professionalism, and an excellent atmosphere.”
Łukasz Grabowski, UG
„No to posferowane 😉 Dziś szkoleniowo z Real Research w Krakowie. Bardzo profesjonalnie, praktycznie, sferycznie. Polecam”
Konrad K., WB UW
"The training turned out to be extremely valuable. I gained knowledge about 3D culture techniques using LifeGel, and the Real Research team was very helpful, answering my questions and supporting me in optimizing the culture conditions for the specific cell types I work with. As a result, my experiments are now better tailored to individual needs and demonstrate greater reproducibility."
Dr Paulina Nastały, GUMed