Technology
A 3D cell culture can be just as easy to obtain as a 2D cell culture!
Cell cultures are one of the most commonly used research tools. We understand that scientists need ready-to-use products for cell cultures and so LifeGel is supplied on standard cell culture plates. All you have to do is open the plate and start to use it!
LifeGel 3D cell culture
LifeGel for 3D cell culture is a ready-to-use plate containing protein-base hydrogel. Cells seeded on the surface of LifeGel can form three-dimensional (3D) structures, like spheroids. LifeGel is a unique product on the market because of its spectacular properties.
Our body is 3D so the research should be too!
2D cell culture – current standard
3D cell culture – LifeGel technology
A layer of LifeGel is placed in a dish, and the cells are then seeded on the surface of the LifeGel where they form 3D structures/spheroids. The cells can grow in multiple layers, migrate within the hydrogel and interact with each other, which recreates the natural environment in which they originally live.
LifeGel is a hydrogel used in 3D cell cultures
LifeGel can be produced in different variations – with different parameters of key characteristics such as hardness, density or elasticity. We have conducted many experiments to find the most suitable combination of these characteristics to fit particular experiments and cell cultures. We know how important it is to have well-matched parameters for optimal cell growth.
Characteristics of LifeGel
Protein-based
Batch to batch repeatable
Definded hydrogel stiffness
Easily digestiable
Customisable for different cells and assays
Supply for small and large users
Compatible with standard imaging platforms
Growth factor free
Comparison of 2D and 3D cell culturing:
Cell cultures are a widely used in vitro tool for understanding cell biology, tissue morphology and disease mechanisms, as well as drug effects, protein production and tissue engineering development. Most cell research is based on experiments using two-dimensional (2D) in vitro cultures. However, 2D cultures have many limitations, such as disrupting the interaction between the cellular and extracellular environment, as well as changes in cell morphology, polarity and partitioning. These disadvantages have led to the creation of new models that mimic physiological conditions much better. One of these new methods is the development of three-dimensional (3D) cultures using a hydrogel with a structure which resembles the extracellular matrix. Cells grown on hydrogels are in many respects very similar to those grown in vivo, e.g. in terms of morphology, the gene expression and protein profile, and cell communication. Optimizing such conditions in culturing allows a better understanding of the biology of cells, such as cancer cells, and can also facilitate the study of biomarkers and targeted therapies.
Comparison of the cultivation of cells in 2D and 3D conditions: This service consists of plating cells into two 48-well plates, one with and one without LifeGel. The most commonly used tiles are panels from different LifeGels, which means that clients can get a comparison between gels with different density and hardness parameters. The cells are cultivated for a fixed number of days (as required by the client) and the experiment ends with imaging of the cells with a bright-field microscope. The photos are then analyzed and sent to the client in the form of a report. It is possible to carry out additional tests and analyses by prior arrangement. This service applies to both cell lines and primary cells provided by the client and is particularly important for those people who are just starting their experience with 3D cultures and want to compare the two types of cell cultivation.
Sample results from the comparison of 2D and 3D cell culture:
Pancreatic tumor cultured in 2D conditions
Pancreatic tumor cultured in 3D conditions
Breast cancer cultures using LifeGel; Days of breeding: A- 14 days, B- 17 days; C- 20 days