Instructions

Application notes

LifeGel plates preparation for cell based assays

LifeGel Digestion Kit - Harvesting cells spheroids from LifeGel plates

Guidelines for Cell Seeding and Proliferation in 3D LifeGel

CellTiter-Blue Cell Viability Assay for 96- or 384-well LifeGel Plates

CellTiter-Glo Cell Viability Assay for 96- or 384-well LifeGel Plates

Cell Staining on LifeGel Using Vibrant Multicolor Cell Labeling DIO, DiD

Fluorescent Triple Staining Protocol for LifeGel-Cultured Cells (DiO, MitoLite, Hoechst)

Immunofluorescent Staining of Actin, Tubulin, and Nuclei in PANC-1 and HEK293 Cells Cultured

MTT Cell Growth Assay for 96-well LifeGel Plates

LifeGel PANC-1 IC₅₀ Determination Using CellTiter-Blue® Assay and Hoechst

Flow Cytometry Staining Protocol for Cells Isolated from LifeGel-Grown Spheroid Cultures

IHC - Procedure for Preparation and Paraffin Embedding of LifeGel Samples

Instruction Movies

How to perform 3D Cell Culture with LifeGel?

How to digest LifeGel with LifeGel Digestion Kit?

3D Cell Cultures

3D Cell Culture on LifeGel

(Opera Phenix)

Pan-02

Pan-02

3D Cell Culture on LifeGel

(Celloger™)

MDA-MB-231

PANC-1

3D Cell Culture on LifeGel

(July™ Stage)

MDA-MB-231

PANC-1

LifeCube

How to perform 3D Cell Culture with LifeCube?