Benefits of working with us

 

  • Experience in 3D cell culture of various human and animal, primary and immortalized lines, PDX and MSC.
  • Cell culture on our innovative, patented technology LifeGel.
  • Our own method of selecting a perfect hydrogel.
  • The whole process from LifeGel production to providing results and final protocol is undertaken by our specialist R&D team.
  • Provision and analysis of results to meet your project needs and expectations.
  • Protocols supplied for repeating the experiment in your own laboratory.
  • Full documentation (CoA, CoO of reagents, list of used laboratory plastics, etc.) that will help later with regulatory bodies (if required).

Standard services

3D cell culture

Culture your cell line in a specially created environment where cells are permitted to grow and interact with surroundings in all three dimensions. 3D cell culture models closely mimic the microenvironments, cell-to-cell interactions and biological processes that occur in vivo.

 

APPLICATION

 

    • 3D cell culture models
    • Cell behavior studies
    • Cell morphology studies
    • Biochemical processes in cells
    • Drug discovery and development
    • Tissue engineering
    • Regenerative medicine
    • Personalized medicine
PANC-1 cells cultured on LifeGel
PANC-1 cells cultured on LifeGel

You send us your chosen cell line.

3D cell culture in our lab: We will choose a perfect hydrogel for your cell line by seeding cells on panels of LifeGels with different parameters of hydrogel (hardness, density, elasticity). We can perform 3D cell culture with selected or individually developed culture media.

You receive the results of our work: description of microscopic observations with photos, data about cell viability, individually prepared cell culture protocols with suggestions about the best hydrogel for your particular cells. We can carry out other specific assays at your request (e.g. staining, drug sensitivity testing).

Compare 2D and 3D cell culture

See the advantage of 3D cell culture in your research. Performing in parallel monolayer 2D culture and three-dimensional 3D culture of the same cell line we can show you the power of 3D cell culture.

 

APPLICATION

 

    • Study differences between cell morphology, phenotype, proliferation
    • Study differences in cell apoptosis models
    • Study differences in intercellular interactions
    • Study differences in gene and protein expression
    • Cell differentiation
    • Study differences in responses to various chemical treatments (drugs, growth factors etc.)
Pancreatic cancer cell line cultured in 2D conditions and 3D conditions
Pancreatic cancer cell line cultured in 2D conditions and 3D conditions

You send us your chosen cell line.

Cell culture in our lab: We will choose a perfect hydrogel for your cell line and perform parallel 2D and 3D cell culture.

You will get the results of our work: description of microscopic observations of differences in 2D and 3D cell culture (with photos), data about cell viability, individually prepared cell culture protocols with suggestion about the best hydrogel. We can carry out other specific assays at your request (e.g. staining, drug sensitivity testing).

Cell culture on LifeGel microcarriers

LifeGel microcarriers increase the growth surface for cells, allowing for fast, cost-effective multiplication of cells on a production scale. Our method for cell culture on LifeGel microcarriers allows for easy recovery of cells with high viability (~95%) using LifeGel Digestion Kit.

 

APPLICATION

 

    • Industrial cell culture
    • Industrial virus culture
    • Cell culture scale-up process
    • Tissue engineering
    • Regenerative medicine
    • Production of antibody and therapeutic proteins
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You send us your chosen cell line.

Cell culture in our lab: We will develop the best parameters for your cell culture on LifeGel microcarriers. We can perform cell culture with standard or individually developed culture medium.

You will get the results of our work: we will create the protocol for your culture on LifeGel microcarriers. We can perform cell culture to provide scale-up of your cells.

Why us?

 

    • Microcarriers are based on our innovative, patented technology LifeGel
    • Our own method of selecting a perfect microcarrier
    • Scalable process
    • Digested microcarriers yield cells with high viability
    • Long term cultures possible

Drug screening on 3D cell culture

3D cultures much better mimic the in vivo conditions, allow for more precise screening of new drugs, and reduce time-consuming tests on animals or less effective tests on 2D lines.

 

APPLICATION

 

    • Drug discovery
    • Drug development
    • Cancer research
RR-wykresy_5-Fluorouracil_Sensitycropped

You send us your chosen cell line and drug.

3D cell culture in our lab: We will culture your cell line on LifeGel and carry out established tests (e.g. IC50 determinations) using your drug.

You will get the results of our work: description of microscopic observations with photos, data about cell viability , individually prepared assay protocols. We can carry out a variety of other specific assays at your request (e.g. alternative viability assay formats).

Why us?

 

    • Established robust IC50 assay protocols for cell viability and / or DNA content using fluorescence-based assays (Z’ values > 0.7)
    • Open to different 3D cell-based assays for drug screening chosen by the client.

Primary tumor tissue drug screening - in development

Carry out your drug screening in 3D cell cultures derived from primary patient tissue.

 

Compare potency and efficacy of different chemotherapeutic agents towards patient-specific tumor derived cells.

 

APPLICATION

 

    • Drug discovery
    • Advanced drug development
    • Cancer research
    • Personalized medicine
RR-wykresy_Oxaliplatin_Sensitivi

You send us patient biopsy tissue and drugs.

3D cell culture in our lab: We will digest biopsy tissue, culture cells on LifeGel in 3D and carry out established viability and DNA assays using your drugs.

You will get the results of our work: description of microscopic observations with photos, data regarding cell viability and DNA quantitation, individually prepared assay protocols. We can carry out a variety of other specific assays at your request (e.g. alternative viability or cell quantitation assay formats).

Why us?

 

    • Established robust IC50 assay protocols for cell viability and / or DNA content using fluorescence-based assays (Z’ values > 0.7)
    • Open to different 3D cell-based assays for drug screening chosen by the client.

3D PDX culture

Create your PDX (Patient Derived Xenograft) models in 3D and mimic the natural environment of tumor growth.

 

APPLICATION

 

    • Personalized medicine
    • Cancer research
    • Drug screening
PDX 3D cell culture on LifeGel
PDX 3D cell culture on LifeGel

You send us a PDX tissue.

In our lab: We will carry out an mechanical disruption and enzymatic tissue digestion to get single cells. We will culture your PDX on selected LifeGel hydrogels and standard or individually developed culture medium.

You will get the results of our work: description of microscopic observations with photos, data about cell viability, individually prepared PDX culture protocols with suggestions about the best hydrogel. We can carry out other specific assays at your request (e.g. staining, drug sensitivity testing).

3D organoid culture - in development

3D organoid cultures are derived from self-renewing stem cells, which have the functions and architecture of the original tissues. We can culture organoids for a variety of assays.

 

APPLICATION

 

    • Drug screening
    • Tissue engineering
    • Human biological mechanistic studies
Cells culture

You send us your organoids.

In our lab: We will culture your organoids on selected LifeGel hydrogels with your chosen culture medium.

You will get the results of our work: description of microscopic observations with photos, data about cell viability, individually prepared organoid culture protocols with suggestions about the best hydrogel. We can carry out other specific assays at your request (e.g. staining, drug sensitivity testing).

Co-culture cell lines - in development

Co-culture models are mainly used to study the effects of physical cell-cell contacts and factors secreted in this culture, and their effect on cell response.

 

APPLICATION

 

    • Study of cell interactions
    • Tissue engineering
    • Immunotherapy
    • CAR-T research
    • Check point inhibitor research
    • Therapeutic antibody research
CHO with Jurkat T on LifeGel
CHO with Jurkat T on LifeGel

You send us your chosen cell lines.

3D cell culture in our lab: We will choose a perfect hydrogel for your cell lines by seeding cells on panels of LifeGels with different elasticities. We can perform 3D cell culture with standard or individually developed culture medium.

You will get the results of our work: description of microscopic observations with photos, data about cell viability, individually prepared cell culture protocol with suggestion about the perfect hydrogel. We can carry out other specific assays at your request (e.g. staining, drug sensitivity testing).

MSC culture

More and more research is focused on MSC cell culture, mainly due to their ability to differentiate into other cells, such as bone, cartilage and fat cells.

 

Our goal is to provide an effective method for maintaining long-term culture of MSC cells in 3D conditions.

 

APPLICATION

 

    • Regenerative medicine research
    • Tissue engineering
    • Cell-based therapies research
pMSC spheroid staining culture on LifeGel
pMSC spheroid staining culture on LifeGel

You send us your chosen MSC cell lines.

3D cell culture in our lab: We will culture your MSC cells using an optimized LifeGel.

You will get the results of our work: description of microscopic observations with photos, data about cell viability, individually prepared MSC culture protocols. We can carry out other specific assays at your request (e.g. staining, drug sensitivity testing).

A ready-made product for 3D MSC culture is now available on our online store.

LifeGel for spheroidal growth of MSC

Single cell cloning in suspension

When you want to avoid cross-contamination from old cell lines, animal cells or other DNA.

 

APPLICATION

 

    • Cell line development
    • Gene therapy
    • Production of recombinant protein and monoclonal antibody
    • Drug screening
    • Gene functional studies
    • CRISPR-cas9, ZFNs, TALENs
zespół pracowników-92

You choose your cell line (adherent or non-adherent) and send it to us. If necessary, we will adapt your cell line to suspension growth and find the right cell culture media which will be later applicable for GMP manufacturing.

Single clone selection phase: Your cell line now adapted to suspension growth will receive additional supplements for single clone selection. At this stage you have to decide how many cells you want to attempt the selection with – we suggest 1000 single cells to give a high chance of obtaining good working clones.

 

We are using our own proprietary method of establishing clones from single cells.

Obtaining the necessary number of cells from clones – increasing the scale of culture, and reduction of cell selection media to a growth media.

 

Shipment of the cells.

Why us?

 

    • Stable cell line production
    • 3 banks of 106 cells each
    • Duration: usually 6-9 months but varies depending on the client’s exact requirements

Pharmacokinetics research on LifeCube - in development

LifeCube is innovative device which allows you to expose 3D cell structures to more pharmacologically relevant drug dosing regimens.

 

The device recreates the drug concentration profiles recorded in the clinic using programmable pairs of pumps.  Not only can the relevant PK profiles be applied to 3D cells to mimic typical injection or infusion strategies, but multiple dosing schemes over several weeks can be simulated and compared.

 

This platform will help define the most effective chemotherapy strategies for specific cell types based on choice of drug concentration and administration frequency.

 

APPLICATION

 

    • Simulation of drug pharmacokinetics and pharmacodynamics for  3D tumor cell structures
LifeCube long term 3D cell culture - Real Research (1)_Moment

You send us your chosen cell line and drug.

3D cell culture in LifeCube: The programmable LifeCube pumping system can mimic drug pharmacokinetics, allowing evaluation of efficacy with different dosing regimens.

You will get the results of our work: a record of cellular response determined by microscopic observation and cell viability data. We can carry out other specific assays at your request (e.g. staining, DNA quantitation), or optimise dosing frequency and concentration for maximum effect.

Why us?

 

    • Cell culture on our patented LifeGel hydrogel technology.
    • Innovative LifeCube platform.
    • Expertise in setting up and performing 3D cell cultures under drug    flow conditions.
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3D cell culture on LifeBot

LifeBot is  an innovative service which allows you to automate your 3D cell culture.

 

We use our experience in the automation of 3D cell culture to create an individual service for you. We can adapt our device directly to your needs.

 

APPLICATION

 

    • Measurements: pH, oxygenation, nitrogen and other small molecules in the sample flowing through the cell culture
    • Module that allows you to attach a syringe pump
    • Fraction collector
    • Imaging
    • Nutrient flow level monitoring
    • Adapting your 3D culture system to 3D culture with constant medium flow

Share your research goals and needs.

We will create a research model for you and adjust the device. We will implement the research plan.

You will get the results of our work.

Why us?

 

    • 3D cell culture on our patented LifeGel hydrogel technology.
    • Innovative LifeBot platform.
    • Expertise in setting up and performing 3D cell cultures.

Our body is 3D so the research should be too!

We want to make the difference in how people do science!

 The difficult we do immediately. The impossible takes a little longer.