Research of the past years has shown that cells cultured in vitro in three-dimensional (3D) resemble their physiological aspects in vivo much better than cells cultured traditionally on flat surface of tissue culture plates (2D). It is reflected in differential gene expression level, cell morphology and biomarker expression level. As a consequence, 3D cell culture is often the better choice for research and development.
We had developed an unique hydrogel for 3D cell culture, called LifeGel.
LifeGel is protein-based hydrogel, free from any growth factors and highly reproducible. Cells seeded on the LifeGel creates a 3D structures, called spheroids, as represented above.
We had also developed a kit for cell rescue from the hydrogel, called LifeGel Digestion Kit. This kit contains ready-to-use collagenase/hyaluronidase enzyme set especially designed for the digestion of the LifeGel. Digestion kit enables easy cell extract from the 3D cell culture.
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